NanoString’s nCounter platform is complete solution for digitally detection and counting large sets of molecules in a single tube.

For translational researchers studying pathways or hypotheses generated on discovery platforms, the nCounter analysis system is ideal solution.

1. More Genes            : Analyze multiple pathways for up to 800 genes

2. High Sensitivity        : Eliminate sample splitting, minimize amplification - get better data from every cell

3. Digital Counting        : Determine fractional fold changes - eliminate the variability of analog data

4. High Throughput        : Analyze hundreds of samples per day

5. Sample compatible    : Study even difficult sample types, including FFPE samples

The Chemistry and Technology

The nCounter Analysis System utilizes a novel digital color-coded barcode technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity (>1 copy per cell). The technology uses molecular "barcodes" and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction.

Each color-coded barcode represents a single target molecule. Barcodes hybridize directly to your target molecules and can be individually counted without the need for amplification– providing very sensitive digital data.

1. Hybridization
NanoString’s Technology employs two ~50 base probes per mRNA that hybridize in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection